The two-day workshop will focus on a practical approach to surface modification related to sensors.
Students should expect to gain
Students will be expected to complete a 4 page report nd a presentation on the workshop content. Upon completion they will be provided a certificate.
All students should complete a detailed project description of their current research project in the registration form. This information will be used to assign you to the appropriate workgroup.
The following section is a worked proforma:
Title of Project: Attachment of microsomes containing CYP enzymes to a gold chip
Aim: (state the overriding goal of your work ) To bind microsomes to a gold coated sensing chip and assess the kinetic measurement of drug metabolism by cytochrome p450 monoxygenases in response to single drug or multiple drug solutions as minics of in-vivo drug metabolism and potential drug-drug inhibitory interactions.
Why: (state the importance/context of work) To improve the measurement of in-vitro drug metabolism assays while reducing the complexity & run cost of instrumentation. To bring about new assay approaches that are equivalent to that of
Objectives: (more detailed descriptive sentences defining a progression in your project)
1) Define and attempt different methods of chemical or physical attachment to gold surface of liposomes/microsomes. Consider monolayer attachment and covalent linkage to proteins at the microsomes bilipid layer as anchors to the gold; and consider the simplicity and effectiveness of exploitation of rapid surface modification offered by physical absorption to the surface
2) Investigate variables affecting the deposition of the microsomes layer such as tailoring flow rates and microsome concentration while correlating to determine the maximum sensor response for each condition
3) Determine enzymes kinetics and binding kinetics from drug compound binding and hydroxylation. Isolate compound based on specificity to one class of CYP over others when used to modify the sensor surface
4) Compare against standard measures of other kinetic assays and run full assays comparing drug drug interactions.