14th Annual Advanced Imaging Methods Workshop

Berkeley, California
Wednesday, January 25, 2017
Pre-Courses (New!)
This year we will be offering three optional pre-course sessions to get attendees up to speed on a number of important microscopy techniques: Adaptive Optics, Fluorescence Lifetime Imaging, and Förster Resonance Energy Transfer. They will be led by world-renowned experts in their respective fields (see bios below).

These sessions will be held concurrently on Tuesday, January 24th, from 2:00 – 5:00P (except for AO, which will be from 3:00-5:30P), at the Berkeley City Club. Each session will include a short break (beverage and light snack). Registration for the Pre-Course is separate from AIM registration. Space is limited, so register today!  


Adaptive Optics for Biological Microscopy

Joel Kubby, University of California at Santa Cruz
Joel Kubby is the Department Chair of Electrical Engineering in the Baskin School of Engineering at the University of California at Santa Cruz. His research is in the application of adaptive optics for biological imaging. Prior to joining the University of California at Santa Cruz in 2005, he was an Area Manager with the Wilson Center for Research and Technology and a Member of Technical Staff in the Webster Research Center in Rochester New York (1987-2005). Prior to Xerox he was at the Bell Telephone Laboratories in Murray Hill New Jersey working in the area of Scanning Tunneling Microscopy (STM).  

Fluorescence Lifetime Imaging (FLIM)

Wolfgang Becker, Becker-Hickl GmbH

Wolfgang Becker is specialist in time-resolved optical detection techniques. He obtained his PhD in 1979 in Berlin, Germany. Since 1993, he has been the head of Becker & Hickl GmbH in Berlin. His field of interest is the development and application of advanced Time-Correlated Single Photon Counting (TCSPC) techniques. He started to develop multi-dimensional TCSPC techniques in 1989, and is the originator of the TCSPC FLIM technique that is now widely used in laser scanning microscopes. He is author of the bh TCSPC handbook, now out in its 6th edition, and of Advanced Single Photon Counting Techniques (Springer, 2005). Dr. Becker is also the editor of Advanced Single Photon Counting Applications (Springer, 2015). He likes cats, skiing, and beach volleyball.

Förster Resonance Energy Transfer (FRET) Microscopy
Ammasi Periasamy, University of Virginia, WM Keck Center for Cellular Imaging

Dr. Periasamy is an internationally recognized expert in advanced microscopy techniques, particularly in the area of molecular imaging in living cells, tissue and animal. A key area of his research is focused on the design and development of optical methodology including advanced light microscopy techniques to investigate/monitor exogenous and endogenous protein-protein interactions, intravital imaging and monitoring the physical parameters of endogenous molecules in normal versus cancer cells/tissues. Dr. Periasamy is the founder and center director of the internationally known W.M. Keck Center for Cellular Imaging (KCCI; http://www.kcci.virginia.edu/). Dr. Periasamy is one of the pioneers in the development of fluorescence lifetime imaging microscopy (FLIM) for measuring the oscillations in cytosolic and nuclear free calcium in single intact living cells. He developed a 2- and 3-color steady state, confocal, multiphoton, and FLIM based Förster resonance energy transfer (FRET) imaging system for protein localization in living specimens. He has also developed 2-photon intravital imaging. He has published over 150 articles in refereed journals and book chapters. He has given more than 160 invited lectures nationally and internationally. Dr. Periasamy has edited three books, is the chairperson and organizer of an annual International conference on Multiphoton Microscopy in the Biomedical Sciences through SPIE (since 2001), and runs a hands-on training annual workshop on FLIM & FRET Microscopy at the University of Virginia, Charlottesville (since 2002). Dr. Periasamy was elected “Fellow” members of the SPIE Optical Society in 2012. Dr. Periasamy is one of the Nature’s webinar participants on FRET and FLIM.

Stephen Vogel
National Institutes of Health, National Institute on Alcohol Abuse and Alcoholism

Dr. Vogel received his B.S. from the City College of New York in 1978 and his Ph.D. in Biochemistry  and Molecular Biophysics from Columbia University in 1989, where he studied G-proteins in the  nervous systems of Aplysia and squid with Jimmy Schwartz. During a postdoctoral fellowship with  Joshua Zimmerberg at NIDDK and NICHD, he investigated the calcium dependence of exocytosis in  sea urchin eggs and discovered heterogeneity for calcium responses in a population of secretory  vesicles. Dr. Vogel next became an assistant and subsequently a tenured associate professor at the  Medical College of Georgia (1997-2003), where he studied the mechanism of exocytosis - endocytosis  coupling. He joined NIAAA as an investigator in 2003, and became a senior investigator in 2013. His laboratory develops new fiber optic and microscope-based assays using FRET, fluorescence polarization, and fluorescence correlation spectroscopy to study protein-protein interactions in living cells, focusing on the structure and function of CaMKII holoenzyme. Dr. Vogel is also a faculty member for the annual University of Virginia FRET Workshop.
 

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    • Online registration is closed. We are accepting on-site registration with a $25 late fee. Cash or check ONLY
    • Sponsors: please contact Holly Aaron for registration and payment. 
    • Volunteers: please contact Jen-Yi Lee.
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