15th Annual Advanced Imaging Methods Workshop

Berkeley, California
Wednesday, January 24, 2018

The AIM 2018 Agenda is HERE (PDF). 

A one page "Program At-A-Glance" can be found HERE (pdf).
The abstracts can be found here: https://drive.google.com/drive/folders/1jaELbOgD1SnRyFuuTqS1Om_Fy1Vg4T4S?usp=sharing

Organizing Committee
Holly Aaron, CRL Molecular Imaging Center, University of California, Berkeley
Wolfgang Becker, Becker-Hickl GmbH
Xavier Darzacq, Molecular and Cell Biology, University of California, Berkeley
Danielle Jorgens, Electron Microscope Lab, University of California, Berkeley
Markita Landry, Chemical and Biomolecular Engineering, UC Berkeley
Evan Miller, Chemistry and Molecular and Cell Biology, UC Berkeley
Jon Mulholland, Cell Sciences Imaging Facility, Stanford University
Laura Waller, Electrical Engineering and Computer Sciences, UC Berkeley

Speakers
Zamal Ahmed, MD Anderson Cancer Center, Houston
Kimberly Beatty, Oregon Health & Science University
Wolfgang Becker, Becker-Hickl, Germany
Reika Watanabe Castillon, University of California, San Deigo
Jeff Chan, University of Illinois, Urbana-Champaign
Laurent Cognet, Institute of Optics, University of Bordeaux, France
Emilia Entcheva, George Washington University
Thomas Gensch, Research Centre Juelich, Germany
Joseph Huff, Zeiss Microscopy
Jennifer Hunter, University of Rochester, New York
Na Ji, University of California, Berkeley
Sanjay Kumar, University of California, Berkeley
Markita Landry, University of California, Berkeley
Luke Lavis, Janelia Farms, Howard Hughes Medical Institute
Alex Lippert, Southern Methodist University, Dallas
Claudia S. López, Oregon Health Sciences University
Alan Mardinly, University of California, Berkeley
Shalin Mehta, Chan-Zuckerberg Biohub, San Francisco
Yves Mely, University of Strasbourg, France
Mustafa Mir, University of California, Berkeley
Vinod Nair, National Institutes of Health
Ammasi Periasamy, University of Virginia, Charlottsville
Angelika Rueck, University of Ulm, Germany
Lydia Sauer, John A. Moran Eye Center, Salt Lake City
Patrick Schäfer, Children's Hospital of Philadelphia
Martin Schnermann, National Institutes of Health
Marina Shirmanova, Nizhny Novgorod State Medical Academy, Russia
Michael Shribak, Marine Biological Laboratory
Melissa Skala, University of Wisconsin, Madison
Ben Smith, University of California, Berkeley
Lin Tian, University of California, Davis
Andreea Trache, Texas A&M University Health Science Center, College Station
Steven Vogel, Laboratory of Molecular Physiology, National Institutes of Health
Laura Waller, University of California, Berkeley
Horst Wallrabe, University of Virginia, Charlottesville
Tomasz Zal, MD Anderson, Houston


AIM 2018 AGENDA

Wednesday, January 24

 

Introductory Session

8:00

Holly Aaron

Welcome

8:10

Dan Fletcher

Back to Basics: Fundamentals of Light Microscopy

8:40

Ben Smith

Seeing the Light: Fundamentals of Cameras and Detectors

9:15

Wolfgang Becker

The Multi-Dimensional World of TCSPC FLIM

10:00

Break

Fluorescence Lifetime Imaging (FLIM) I

Session Chair: Feather Ives, UC Berkeley

10:30

Thomasz Zal

Interplay of Tumor Oxygenation and Immunity Investigated by Dynamic 2PLM

11:00

Sebastian Tille

Leica SP8 FALCON: FAst Lifetime CONtrast for Functional & Multi-Modal Imaging
FLIM Goes Video Rate!

11:15

Short Break

Mechanobiology Imaging

Session Chair: Jon Mulholland, Stanford University

11:30

Andreea Trache

Light Meets Force: Mechanobiology Studies Using Integrated Optical and Atomic Force Microscopy

12:00

Pascal Odermatt

Mechanical Manipulation of Bacterial Cell Division

12:30

Sanjay Kumar

Characterization of an Intrinsically Disordered and Enzyme-Responsive Protein Brush by Atomic Force Microscopy

1:00

Lunch

Terrace and Julia’s Lounge

2:00 - 3:30

Vendor Fair

Member’s Lounge & Venetian Room

PRIZE DRAWINGS

Correlative Microscopy

Session Chair: Danielle Jorgens, UC Berkeley

3:30

Joseph Huff

Cryo-Confocal Imaging with Airyscan for Correlative Imaging

4:00

Claudia S. López

Correlative Light and Electron Microscopy Methods for 2D and 3D Imaging

4:30

Reika Watanabe Castillon

Revealing Protein Structure in Their Native Context Through Cryo Electron Tomography of Cells

5:00

Vinod Nair

Chicken Soup for the Soul: CLEM on a Budget

5:30

Break

Computational Imaging

Session Chair: Henry Pinkard, UC Berkeley

6:00

Shalin Mehta

Scalable Imaging of Molecular Order

6:30

Laura Waller

Fast 3D Computational Fluorescence Microscopy

7:00

Michael Shribak

Quantitative Orientation-Independent Differential Interference Contrast Microscope for High-Resolution Phase Mapping

7:30

Alan Mardinly

Precise Holographic Manipulation of Neural Ensembles in Behaving Animals

 

 

8:30 - 10:00

Speaker Dinner

(RSVP required)


 

Thursday, January 25

 

 

Florescence Lifetime Imaging (FLIM) II

Session Chair: Wolfgang Becker, Becker-Hickl

8:00

Alzbeta Chorvatova

Resolving Individual Autofluorescence Components

8:30

Patrick Schäfer

Unscrambling Metabolic Information From NADH Autofluorescence

9:00

Marina Shirmanova

FLIM/PLIM for Multiparametric Study of Tumor Biology and Drug Response

9:30

Angelika Rueck

Luminescence Lifetime Imaging Microscopy (LIM) to Follow Up Cell Metabolism and Oxygen Consumption During Theranostic Applications

 

10:00 - 11:00

Vendor Fair

Member’s Lounge & Venetian Room

PRIZE DRAWINGS

 

 

 

Photons in the Brain

Session Chair: Ian McFarlane, UC Berkeley

11:00

Markita Landry

Exciton Engineering for Brain Imaging of Modulatory Neurotransmitters

11:30

Lin Tian

Light Up the Brain with Genetically Encoded Indicators of Neural Activity

12:00

Luke Lavis

Designing Brighter Dyes for Advanced Fluorescence Microscopy

12:30

Laurent Cognet

Nanoscale Imaging of Live Brain Tissue by Single Molecule Microscopy

 

1:00

Lunch

Terrace and Julia’s Lounge

 

 

 

 

 

 

 

Fluorescence Lifetime Imaging (FLIM) III

Session Chair: Ana Robles, UC Berkeley

2:00

Ammasi Periasamy

Fluorescence Lifetime Redox Ratio (FLIRR): A New Approach to Investigate NADH, FAD and Tryptophan in Live Cancer Cells

2:30

Yves Mely

Monitoring HIV-1 Nucleocapsid Protein During the Early Steps of Infection by Advanced Fluorescence Microscopy Techniques

3:00

Melissa Skala

Optical Imaging of Immune Cell Metabolism

3:30

Stephen Vogel

Ultra-Fast Long-Distance Energy Transfer Between Fluorescent Proteins

4:00

Break

Designing Probes for Microscopy

Session Chair: Evan Miller, UC Berkeley

4:30

Bruce Cohen

Low Fluence Multiphoton Imaging with Alloyed Lanthanide Nanocrystals

5:00

Alexander Lippert

Recent Progress in Chemiluminescence Imaging

5:30

Kimberly Beatty

A New Technology for Tracking Cellular Proteins at High Resolution

6:00

Jeff Chan

From Light to Sound: Photoacoustic Probes for Non-invasive In Vivo Imaging

6:30

Martin Schnermann

Harnessing Cyanine Reactivity for Imaging and Drug Delivery

7:00 - 9:00

Poster Session & Reception

Drawing Room, 1st Floor BCC


 

Friday, January 26

 

 

Lightsheet Microscopy

Session Chair: Xavier Darzacq, UC Berkeley

8:00

Liang Gao

Imaging Multicellular Specimens with Tiling Light Sheet Selective Plane Illumination Microscopy

8:15

Mustafa Mir

Imaging Transcriptional Dynamics at the Single Molecule Level in Living Embryos

8:45

Loic Royer

Pushing the Limits of Light-sheet Microscopy with Adaptive Imaging and Machine Learning

9:15

Na Ji

Imaging Neural Activity Over Large Volumes at High Spatiotemporal Resolution

9:45

Sandrine Lévêque-Fort

Supercritical Angle Fluorescence Emission : An Alternative Access to Axial Resolution in Microscopy

10:15

Break

10:30 - 12:30

Demos

Demos on and off-site (see demonstration listings for locations and descriptions)

 

1:00

Lunch

BCC, Terrace and Julia’s Lounge

 

 

FLIM IV – Clinical Imaging

Session Chair: Wolfgang Becker, Becker-Hickl

2:00

Hauke Studier

Non-Invasive Metabolic Imaging of Melanoma Progression

2:30

Lydia Sauer

Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO)

3:00

Jennifer Hunter

Cellular-Scale Fluorescence Lifetime Imaging of the Living Eye

3:30

Karsten König

Multiphoton Tomography for Stem Cell Research, Cornea Transplant Diagnostics, and Skin Cancer Imaging

4:00

Emilia Entcheva

Massively Parallel All-Optical Cardiac Electrophysiology

4:30

Break

FLIM V – Innovations

Session Chair: Annette Chan, San Francisco State University

 

5:00

Horst Wallrabe

Three-Photon FLIM-FRET Microscopy - Exploring the Merits of Different ‘Apparent E%’ Calculation Models for Autofluorescent Tryptophan (Donor) – NAD(P)H (Acceptor)

5:30

Zamal Ahmed

The Contribution of FLIM in Mechanistic Understanding of FGFR2 Signaling

6:00

Thomas Gensch

Fluorescence Lifetime Imaging (FLIM) for Intracellular pH Determination and the Role of the Photon Number

6:30

Wolfgang Becker

Ultra-Fast Hybrid Detectors Improve NAD(P)H FLIM

7:00 - 9:00

Farewell Reception

Drawing Room, 1st Floor BCC

 

 







 

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